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. 2016 Sep 2;21(9):1169. doi: 10.3390/molecules21091169

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© 2016 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of atractylodin on the release of IL-6 (A) and the expression of TNF-α, CSF2, IL-4, and IL-6 mRNA (B,C) in PMA plus A23187-stimulated HMC-1 and cell was treated with the indicated concentrations of atractylodin for 0.5 h prior to being incubated with PMA (50 nM) plus A23187 (1 μM) for 24 h and detected by ELISA, RT-PCR, and real-time RT-PCR, analysis as described in Materials and Methods. Monte10: montelukast 10 μM. Statistical significance: * p < 0.05 as compared to the PMA plus A23187 treated group. ** p < 0.005 as compared to the PMA plus A23187 treated group. Values shown are the mean ± S.E. of duplicate determinations from three separate experiments.

Effect of atractylodin on the release of IL-6 (A) and the expression of TNF-α, CSF2, IL-4, and IL-6 mRNA (B,C) in PMA plus A23187-stimulated HMC-1 and cell was treated with the indicated concentrations of atractylodin for 0.5 h prior to being incubated with PMA (50 nM) plus A23187 (1 μM) for 24 h and detected by ELISA, RT-PCR, and real-time RT-PCR, analysis as described in Materials and Methods. Monte10: montelukast 10 μM. Statistical significance: * p < 0.05 as compared to the PMA plus A23187 treated group. ** p < 0.005 as compared to the PMA plus A23187 treated group. Values shown are the mean ± S.E. of duplicate determinations from three separate experiments.