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. 2016 Sep 2;21(9):1169. doi: 10.3390/molecules21091169

Figure 6.

Figure 6

Effect of atractylodin and PF-02341066 on the phosphorylation of MAPKs (A) and the expression of IL-6 mRNA (B) in PMA plus A23187-stimulated HMC-1. (A) Cells were treated with or without atractylodin and PF-02341066 (1 μM) for 0.5 h and then treated with PMA plus A23187 for 0.5 h. Whole cell lysates were then analyzed by Western blot; (B) Cell was treated with atractylodin for 0.5 h prior to being incubated with PMA (50 nM) plus A23187 (1 μM) for 24 h and detected by real-time RT-PCR analysis as described in Materials and Methods. Statistical significance: * p < 0.05 as compared to the PMA plus A23187 treated group.