Table 1.
Spot No. # | Identified Protein & | Accession * | Organism ¥ | Mass (kDa) £ | S ± | Peptide Sequence ≠ |
---|---|---|---|---|---|---|
1 | Angiotensin- like peptide 2 | ANGT2_BOTJA | Bothrops jararaca | 1.04 | 44 | DRVYIHPF (1046.5816) |
2 | H3 metalloproteinase precursor 1 | VM1H3_DEIAC | Vipera ammotydes ammotydes | 70.7 | 48 | NPCQIYYTPR (1311.7225) |
3 | l-amino acid oxidase | OXLA_BOTPC | Bothrops pictus | 56.7 | 102 | SAGQLYEESLR (1252.6349) |
OXLA_BOTCO | Bothrops cotiara | 1.8 | 49 | NPLEECFR (1252.6575) | ||
OXLA_MACLB | Macrovipera lebetina | 12.5 | 58 | PMF SC 40.2% | ||
OXLA_BOTJR | Bothrops jararacussu | 56.7 | 59 | FWEDDGIHGGK (1260.6088) | ||
OXLA_VIPBB | Vipera berus berus | 10.3 | 68 | ADDICNPLEECFR (1493.7661) | ||
OXLA_BOTJA | Bothrops jararacussu | 56.5 | 50 | HIDDIFAYEK (1137.5991) | ||
OXLA_BOTPC | Bothrops pictus | 56.7 | 101 | SAGQLYEESLR (1252.7064) | ||
OXLA_BOTIN | Bothrops insularis | 5.3 | 37 | ADDKNPLEECFR (1493.7653) | ||
4a | Serine protease VLSp-3 | VSP3_MACLB | Macrovipera lebetina | 29 | 25 | TSTHIAPLSLPSSPPSVGSVCR (2250.4505) |
4b | Snake venom serine protease nikobin | VSP_VIPNI | Vipera nikolskii | 28.8 | 64 | PMF SC 25.7% |
53 | CQGVHPELPAK (1235.6438) | |||||
55 | VVCAGIWQGGK (1174.6110) | |||||
96 | VILPDVPHCANIEIIK (1831.0636) | |||||
36 | EYTMWDK (972.4713) | |||||
5 | Beta- fibrinogenase brevinase | VSPB_GLOBL | Gloydius blomhoffi | 26.3 | 56 | VIGGDECNINEHR (1512.7806) |
VSPBF_MACLB | Macrovipera lebetina | 28.2 | 26 | FFCLSSK (888.4284) | ||
Thrombin-like enzyme crotalase | VSPCR_CROAD | Crotalus adamanteus | 30.1 | 36 | WDKDIMLIR (1189.6561) | |
Venom serine proteinase-like protein 2 | VSP2_MACLB | Macrovipera lebetina | 29.5 | 70 | IMGWGTITTTK (1208.6420) | |
134 | TLCAGILQGGIDSCK (1592.7767) | |||||
6 | Cysteine rich venom protein | CRVP_VIPBE | Vipera berus | 27.4 | 73 | MEWYPEAAANAER (1537.6894) |
53 | PMF SC 24.7% | |||||
74 | PMF SC 36.4% | |||||
51 | KPEIQNEIIDLHNSLR (1919.0970) | |||||
7 | Snake venom metalloproteinase VMP1 | VM1V1_AGKPL | Agkistrodon piscivorus leucostoma | 47.1 | 58 | NPQCILNKPLR (1352.7017) |
Zinc metalloproteinase/disintegrin | VM2L2_MACLB | Macrovipera lebetina | 47.1 | 58 | NPQCILNQPL (1352.7017) | |
8a | Snaclec rhinocetin submit beta | SLRB_BITRH | Bitis rhinoceros | 18.7 | 59 | TTDNQWLR (1033.6040) |
8b | Snaclec A14 | SLAE_MACLB | Macrovipera lebetina | 18 | 39 | TSADYVWIGLWNQR (1708.9104) |
8c | Snaclec B6 | SLB6_MACLB | Macrovipera lebetina | 15.3 | 43 | ANLVWIGLR (1041.6803) |
8d | Snaclec echicetin subunit alpha | SLA_ECHCA | Echis carinatus | 16.1 | 37 | TWDEAEKFCNK (1427.6446) |
8e | Snaclec 1 | SL1_ECHCS | Echis carinatus sochureki | 17.1 | 35 | GSHLVSLHNIAEADFVVK (1936.0465) |
8f | Snaclec rhodocetin subunit alpha | SLEA_CALRH | Calloselasma rhodostoma | 15.9 | 47 | TWEEAER (920.46147) |
8f | Snaclec A13 | SLAD_MACLB | Macrovipera lebetina | 15.6 | 26 | DQDCLPGWSFYEGHCYK (2161.9310) |
8g | Snaclec jerdonibitin submit alpha | SLA_PROJR | Protobothrops jerdoni | 18.1 | 45 | TWEDAER (906.4700) |
9 | Acidic phospholipase ammodytin I1 | PA2A1_VIPAA | Vipera ammodytes ammodytes | 16.2 | 56 | PMF SC 30.4% |
Acidic phospholipase A2 PLA-1 | PA2A1_ERIMA | Eristicophis macmahoni | 14.3 | 58 | PMF SC 36.4% | |
Acidic phospholipase A2 PL1 | PA2A1_VIPRE | Viprea renardi | 16.2 | 72 | CCFVHDCCYGR (1533.4506) | |
10 | Neutral phospholipase A2 ammodytoxin I2 | PA2N_DABRR | Vipera ammodytes ammodytes | 16.1 | 56 | PMF SC 30% |
11 | Basic phospholipase A2 ammodytoxin C | PA2BA_VIPAA | Vipera ammodytes ammodytes | 15.4 | 28 | AAAICFR (808.4193) |
Basic phospholipase A2 Pla2Vb | PA2B_VIPBB | Vipera berus berus | 15.7 | 48 | HICECDR (989.4389) | |
Basic phospholipase A2 vurtoxin | PA2B_VIPRE | Vipera renardi | 16.4 | 31 | YYPDFLCK (1105.5024) | |
Basic phospholipase A2 | PA2B3_DABRR | Daboia russelii | 14.4 | 142 | CCFVHDCCYGNLPDCNPK (2315.8464) |
# Spot numbering was the same as in Figure 1 and Figure 2; & Protein name in database; * Database accession number of homologous proteins; ¥ Organism from which protein originates; £ The mass of molecule; ± Protein identification was performed using the Mascot search with probability based Mowse score. Ions score was −10 × log(P), where P was the probability that the observed match was a random event. ≠ Peptide sequence derived from LIFT analysis. Identification of proteins by MS/MS method was conducted by comparing obtained sequences with sequences from database. In brackets mass of precursor ion. In the case of PMF, identification SC amino acid sequence coverage for the identified proteins. In the PMF identification case, the highest score and SC are shown.