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. 2016 Aug 22;21(8):1071. doi: 10.3390/molecules21081071

Figure 4.

Figure 4

Effect of MDHB on TBHP-induced ROS accumulation in SH-SY5Y cells. SH-SY5Y cells were treated with MDHB (2, 4, or 8 μM) for 4 h before exposure to 40 μM TBHP for another 24 h. Intracellular ROS levels were measured via incubation with DCFH-DA fluorescent dye and analyzed using flow cytometry. (A) Control group; (B) 40 μM THBP group; (C) 2 μM MDHB + TBHP group; (D) 4 μM MDHB + TBHP group; and (E) 8 μM MDHB + TBHP group; The statistical result of DCFH-DA staining (F) showed that MDHB (2, 4, 8 μM) can reduce remarkably TBHP-induced ROS accumulation in SH-SY5Y cells. Values represent the mean ± S.E.M, ## p < 0.01 versus the control group. * p < 0.05 versus the TBHP group. ** p < 0.01 versus the TBHP group (n = 3).