Figure 2.

Neuroprotective activity of compounds against cell damage induced by chemical hypoxia and glucose deprivation. PC12 cells were cultured in oxygen and glucose deprivation conditions (OGD), specifically in the absence of glucose and in the presence of 600 µM of potassium cyanide (KCN) for 1 h, and then, normal culture medium was reperfused to the cells. After 4 h of the reperfusion start, some PC12 cells were treated with solvent control (DMSO), solasodine (SL) or different concentrations of the S15 derivative. After 20 h of exposition to the reperfusion damage and treatment, cellular viability was determined by the MTT assay. Data are represented as the percent with respect to the untreated cells, representing the highest cell viability. Asterisks indicate statistical differences between the treatment and respective control condition (*** p < 0.001) according to one-way ANOVA and Tukey’s post-hoc test.