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. 2016 Jul 14;21(7):913. doi: 10.3390/molecules21070913

Table 4.

Precision and accuracy of the determination of eight alkaloids in rat plasma (n = 18, 6 replicates per day for 3 days).

Compounds Spiked Concentration (ng/mL) Measured CONC (ng/mL) Accuracy (%) Intra-Day Precision (%) Inter-Day Precision (%)
Tetrahydropalmatine 0.5 0.5 ± 0.1 −1.0 17.6 18.8
2.0 2.1 ± 0.3 −3.4 13.6 3.7
50.7 53.1 ± 6.9 −3.0 12.9 12.9
1622 1759 ± 172 8.4 9.1 13.8
Palmatine 0.1 0.1 ± 0.02 8.5 18.5 15.6
0.4 0.5 ± 0.07 11.2 13.4 11.9
10.7 11.1 ± 1.5 3.4 14.2 10.3
342 368 ± 41.8 7.5 11.8 6.7
Magnoflorine 1.1 1.3 ± 0.1 14.1 11.7 6.6
4.3 4.3 ± 1.1 7.1 14.3 4.7
108 116 ± 13.8 7.5 12.0 10.3
3456 3817 ± 485 10.4 12.5 14.4
Columbamine 0.6 0.6 ± 0.04 −14.4 14.9 14.2
2.2 2.5 ± 0.3 9.8 14.3 3.5
55.8 53.9 ± 6.7 −3.4 12.3 13.5
1784 1713 ± 193 −4.0 11.0 13.4
Berberine 0.1 0.1 ± 0.01 −2.9 10.0 17.8
0.4 0.5 ± 0.1 5.5 14.3 6.3
11.1 10.7 ± 1.0 −7.3 8.7 12.4
354 317 ± 30.7 −10.4 10.0 6.8
Worenine 0.6 0.6 ± 0.1 −0.1 16.8 13.9
2.2 2.2 ± 0.3 −4.3 14.0 10.3
55.5 51.3 ± 6.1 −7.4 12.0 12.0
1776 1715 ± 90.6 −3.4 4.9 7.6
Berberrubine 1.1 1.1 ± 1.1 0.8 14.3 9.2
4.4 5.3 ± 0.6 14.1 11.6 8.4
111 112 ± 14.0 1.5 13.1 6.2
3536 3949 ± 437 11.7 11.3 9.4
Coptisine 0.2 0.2 ± 0.03 13.4 13.8 1.8
0.8 0.8 ± 0.1 2.1 12.7 9.9
20.0 22.4 ± 2.6 11.8 11.9 9.0
640 555 ± 70.3 −13.3 13.1 8.4