Table 4.

Precision and accuracy of the determination of eight alkaloids in rat plasma (n = 18, 6 replicates per day for 3 days).

Compounds	Spiked Concentration (ng/mL)	Measured CONC (ng/mL)	Accuracy (%)	Intra-Day Precision (%)	Inter-Day Precision (%)
Tetrahydropalmatine	0.5	0.5 ± 0.1	−1.0	17.6	18.8
	2.0	2.1 ± 0.3	−3.4	13.6	3.7
	50.7	53.1 ± 6.9	−3.0	12.9	12.9
	1622	1759 ± 172	8.4	9.1	13.8
Palmatine	0.1	0.1 ± 0.02	8.5	18.5	15.6
	0.4	0.5 ± 0.07	11.2	13.4	11.9
	10.7	11.1 ± 1.5	3.4	14.2	10.3
	342	368 ± 41.8	7.5	11.8	6.7
Magnoflorine	1.1	1.3 ± 0.1	14.1	11.7	6.6
	4.3	4.3 ± 1.1	7.1	14.3	4.7
	108	116 ± 13.8	7.5	12.0	10.3
	3456	3817 ± 485	10.4	12.5	14.4
Columbamine	0.6	0.6 ± 0.04	−14.4	14.9	14.2
	2.2	2.5 ± 0.3	9.8	14.3	3.5
	55.8	53.9 ± 6.7	−3.4	12.3	13.5
	1784	1713 ± 193	−4.0	11.0	13.4
Berberine	0.1	0.1 ± 0.01	−2.9	10.0	17.8
	0.4	0.5 ± 0.1	5.5	14.3	6.3
	11.1	10.7 ± 1.0	−7.3	8.7	12.4
	354	317 ± 30.7	−10.4	10.0	6.8
Worenine	0.6	0.6 ± 0.1	−0.1	16.8	13.9
	2.2	2.2 ± 0.3	−4.3	14.0	10.3
	55.5	51.3 ± 6.1	−7.4	12.0	12.0
	1776	1715 ± 90.6	−3.4	4.9	7.6
Berberrubine	1.1	1.1 ± 1.1	0.8	14.3	9.2
	4.4	5.3 ± 0.6	14.1	11.6	8.4
	111	112 ± 14.0	1.5	13.1	6.2
	3536	3949 ± 437	11.7	11.3	9.4
Coptisine	0.2	0.2 ± 0.03	13.4	13.8	1.8
	0.8	0.8 ± 0.1	2.1	12.7	9.9
	20.0	22.4 ± 2.6	11.8	11.9	9.0
	640	555 ± 70.3	−13.3	13.1	8.4