Table 4.
Precision and accuracy of the determination of eight alkaloids in rat plasma (n = 18, 6 replicates per day for 3 days).
Compounds | Spiked Concentration (ng/mL) | Measured CONC (ng/mL) | Accuracy (%) | Intra-Day Precision (%) | Inter-Day Precision (%) |
---|---|---|---|---|---|
Tetrahydropalmatine | 0.5 | 0.5 ± 0.1 | −1.0 | 17.6 | 18.8 |
2.0 | 2.1 ± 0.3 | −3.4 | 13.6 | 3.7 | |
50.7 | 53.1 ± 6.9 | −3.0 | 12.9 | 12.9 | |
1622 | 1759 ± 172 | 8.4 | 9.1 | 13.8 | |
Palmatine | 0.1 | 0.1 ± 0.02 | 8.5 | 18.5 | 15.6 |
0.4 | 0.5 ± 0.07 | 11.2 | 13.4 | 11.9 | |
10.7 | 11.1 ± 1.5 | 3.4 | 14.2 | 10.3 | |
342 | 368 ± 41.8 | 7.5 | 11.8 | 6.7 | |
Magnoflorine | 1.1 | 1.3 ± 0.1 | 14.1 | 11.7 | 6.6 |
4.3 | 4.3 ± 1.1 | 7.1 | 14.3 | 4.7 | |
108 | 116 ± 13.8 | 7.5 | 12.0 | 10.3 | |
3456 | 3817 ± 485 | 10.4 | 12.5 | 14.4 | |
Columbamine | 0.6 | 0.6 ± 0.04 | −14.4 | 14.9 | 14.2 |
2.2 | 2.5 ± 0.3 | 9.8 | 14.3 | 3.5 | |
55.8 | 53.9 ± 6.7 | −3.4 | 12.3 | 13.5 | |
1784 | 1713 ± 193 | −4.0 | 11.0 | 13.4 | |
Berberine | 0.1 | 0.1 ± 0.01 | −2.9 | 10.0 | 17.8 |
0.4 | 0.5 ± 0.1 | 5.5 | 14.3 | 6.3 | |
11.1 | 10.7 ± 1.0 | −7.3 | 8.7 | 12.4 | |
354 | 317 ± 30.7 | −10.4 | 10.0 | 6.8 | |
Worenine | 0.6 | 0.6 ± 0.1 | −0.1 | 16.8 | 13.9 |
2.2 | 2.2 ± 0.3 | −4.3 | 14.0 | 10.3 | |
55.5 | 51.3 ± 6.1 | −7.4 | 12.0 | 12.0 | |
1776 | 1715 ± 90.6 | −3.4 | 4.9 | 7.6 | |
Berberrubine | 1.1 | 1.1 ± 1.1 | 0.8 | 14.3 | 9.2 |
4.4 | 5.3 ± 0.6 | 14.1 | 11.6 | 8.4 | |
111 | 112 ± 14.0 | 1.5 | 13.1 | 6.2 | |
3536 | 3949 ± 437 | 11.7 | 11.3 | 9.4 | |
Coptisine | 0.2 | 0.2 ± 0.03 | 13.4 | 13.8 | 1.8 |
0.8 | 0.8 ± 0.1 | 2.1 | 12.7 | 9.9 | |
20.0 | 22.4 ± 2.6 | 11.8 | 11.9 | 9.0 | |
640 | 555 ± 70.3 | −13.3 | 13.1 | 8.4 |