Table 2.
Quantification data of trastuzumab signature peptide (FTISADTSK) in human serum batch; calibration curve (CC) extends from 5 µg/mL to 500 µg/mL; their corresponding accuracy percentage and R2 = 0.99 was observed.
| Sample Name (CC) | Analyte Peak Area | Analyte RT (min) | IS Peak Area | IS Retention Time (min) | Area Ratio | Analyte Conc (µg/mL) | Calculated Conc (µg/mL) | Accuracy (%) |
|---|---|---|---|---|---|---|---|---|
| STD_8 | 2826503 | 6.058 | 513894 | 6.056 | 5.500 | 501.600 | 495.319 | 98.7 |
| STD_7 | 2668953 | 6.077 | 554130 | 6.075 | 4.816 | 451.440 | 433.648 | 96.1 |
| STD_6 | 2174781 | 6.051 | 526182 | 6.048 | 4.133 | 375.598 | 372.009 | 99.0 |
| STD_5 | 1201036 | 6.075 | 436818 | 6.074 | 2.750 | 251.651 | 247.200 | 98.2 |
| STD_4 | 432522 | 6.081 | 506952 | 6.079 | 0.853 | 75.495 | 76.145 | 100.9 |
| STD_3 | 251462 | 6.140 | 908537 | 6.139 | 0.277 | 25.668 | 24.152 | 94.1 |
| STD_2 | 69119 | 6.123 | 521644 | 6.120 | 0.133 | 10.267 | 11.138 | 108.5 |
| STD_1 | 44541 | 6.118 | 623461 | 6.116 | 0.071 | 5.134 | 5.630 | 109.7 |