Figure 2.

Effect of DBMB on mRNA expression level of inflammatory mediators, and the transcriptional activation of NF-κB. (a) mRNA expression levels of pro-inflammatory mediators in RAW264.7 cells treated with DBMB and LPS (1 μg/mL); (b) NF-κB promoter activity was tested by luciferase assay under PMA (100 nM) treatment condition with DBMB on NF-κB reporter gene and β-galactosidase gene (as a control) transfected HEK293 cells; (c,d) Luciferase reporter activity was determined by NF-κB luciferase reporter gene assay, in FLAG-MyD88 or CFP-TRIF plasmid transfected HEK293 cells; (e) The translocation level of NF-κB transcription factor subunits, p65 and p50, was determined by immunoblotting with antibodies against phospho- or total proteins in the nuclear fraction of LPS-treated RAW264.7 cells. All values (a–d) are presented as means ± SDs. * p < 0.05 and ** p < 0.01 compared with controls.