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. 2016 Jul 18;21(7):933. doi: 10.3390/molecules21070933

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© 2016 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Comparison of circulating CD4⁺CD25^highFoxP3⁺ and CD4⁺CD25^highCD127⁻ Treg lymphocytes percentages between asthmatic patients with or without steroid treatment, and control subjects. Representative dot plots and gating strategy are shown for the (A) CD4⁺CD25^highFoxP3⁺ and (B) CD4⁺CD25^highCD127⁻ Treg lymphocytes. Briefly, the CD4⁺CD25⁺ cells were gated from total lymphocytes, and then the FoxP3⁺ or CD127⁻ cells were gated from the top 20% high CD25⁺ cells of CD4⁺CD25⁺ cells; (C,D) The proportion of circulating Treg lymphocytes in total lymphocytes from asthmatic patients with or without steroid treatment, and control subjects were determined by flow cytometry. Results are presented as box and whisker plots with median (interquartile range). Statistical significances were indicated by * p < 0.05 and ** p < 0.01 (Mann-Whitney U test). S-asthma: steroid-treated asthmatic patients, NS-asthma: non-steroid-treated asthmatic patients, control: control subjects.

Comparison of circulating CD4⁺CD25^highFoxP3⁺ and CD4⁺CD25^highCD127⁻ Treg lymphocytes percentages between asthmatic patients with or without steroid treatment, and control subjects. Representative dot plots and gating strategy are shown for the (A) CD4⁺CD25^highFoxP3⁺ and (B) CD4⁺CD25^highCD127⁻ Treg lymphocytes. Briefly, the CD4⁺CD25⁺ cells were gated from total lymphocytes, and then the FoxP3⁺ or CD127⁻ cells were gated from the top 20% high CD25⁺ cells of CD4⁺CD25⁺ cells; (C,D) The proportion of circulating Treg lymphocytes in total lymphocytes from asthmatic patients with or without steroid treatment, and control subjects were determined by flow cytometry. Results are presented as box and whisker plots with median (interquartile range). Statistical significances were indicated by * p < 0.05 and ** p < 0.01 (Mann-Whitney U test). S-asthma: steroid-treated asthmatic patients, NS-asthma: non-steroid-treated asthmatic patients, control: control subjects.