. 2016 May 16;21(5):643. doi: 10.3390/molecules21050643

Table 2.

Neurotoxicity and Cu(II) chelating activity of the studied compounds and positive control Na₂EDTA.

Compound	Cell Viability IC₅₀ (µM) ^a	Cu(II)-Chelating Activity (%) ^b
3	10.89 ± 1.63	5.37 ± 0.16
4	23.66 ± 3.54	15.07 ± 1.32
5	n.d ^c	19.73 ± 0.30
Na₂EDTA	-	17.10 ± 1.44

^a Concentration of compound resulting in 50% inhibition of cell viability after 24 h treatment. The values are mean ± SD of at least two independent experiments; ^b Chelating activity observed at 20 µM concentration of compound and Na₂EDTA as positive control. The values are mean ± SD of at least two independent experiments; ^c n.d. = IC₅₀ not determined because less than 50% inhibition was observed at the highest concentration tested (40 µM).