Table 3.
Intra- and inter-day precision and accuracy for the determinations for the three compounds.
| Nominal Conc. (ng/mL) | Intra-Day | Inter-Day | ||||
|---|---|---|---|---|---|---|
| Observed Conc. (ng/mL) | Precision, RSD (%) | Accuracy, Bias (%) | Observed Conc. (ng/mL) | Precision, RSD (%) | Accuracy, Bias (%) | |
| Scoparone | ||||||
| 1 | 1.02 ± 0.14 | 14.08 | 1.79 | 1.03 ± 0.13 | 12.40 | 3.12 |
| 5 | 5.02 ± 0.49 | 9.78 | 0.32 | 5.20 ± 0.64 | 12.31 | 3.94 |
| 10 | 10.08 ± 0.77 | 7.65 | 0.81 | 10.40 ± 1.04 | 10.02 | 4.02 |
| 50 | 50.94 ± 3.50 | 6.87 | 1.89 | 51.91 ± 4.45 | 8.57 | 3.82 |
| 100 | 99.39 ± 4.71 | 4.74 | –0.61 | 98.12 ± 3.49 | 3.56 | –1.88 |
| Geniposide | ||||||
| 5 | 5.49 ± 0.25 | 4.53 | 9.83 | 5.57 ± 0.34 | 6.09 | 11.31 |
| 10 | 9.95 ± 0.46 | 4.61 | –0.54 | 10.17 ± 0.55 | 5.45 | 1.73 |
| 50 | 48.91 ± 1.17 | 2.40 | –2.18 | 49.93 ± 1.63 | 3.26 | –0.13 |
| 100 | 101.32 ± 3.08 | 3.04 | 1.32 | 100.35 ± 2.98 | 2.97 | 0.35 |
| 500 | 501.13 ± 18.27 | 3.64 | 0.23 | 519.20 ± 25.51 | 4.91 | 3.84 |
| 1000 | 1090.58 ± 44.58 | 4.09 | 9.06 | 1107.45 ± 43.45 | 3.92 | 10.74 |
| Rhein | ||||||
| 5 | 5.05 ± 0.43 | 8.43 | 0.95 | 4.53 ± 0.29 | 6.45 | –9.34 |
| 10 | 9.39 ± 0.74 | 7.93 | –6.10 | 9.92 ± 0.41 | 4.13 | –0.84 |
| 50 | 50.57 ± 4.25 | 8.41 | 1.15 | 53.08 ± 6.22 | 11.72 | 6.17 |
| 100 | 99.17 ± 1.67 | 1.69 | –0.83 | 103.37 ± 4.28 | 4.14 | 3.37 |
| 500 | 423.21 ± 9.03 | 2.13 | –15.36 | 492.91 ± 63.74 | 12.93 | –1.42 |
Data are expressed as the means ± standard deviations (n = 5).