Figure 2.

Cytotoxicity of Hcy and icariin measured by LDH assay. Cortical neurons were treated with Hcy (A), Icariin (B) and Hcy plus icariin (C) and performed LDH assay. The treatment duration for Hcy or Icariin was 12 h. In the co-treatment, cells were pretreated 1 h before the addition of Hcy. Results were quantified and expressed as fold of control. Data represents mean ± SE from at least three independent experiments. The significance of differences among treatment groups was determined by one-way ANOVA. *: p < 0.05 compared to control. “#”: p < 0.05. “+” and “-”: with and without the compound.