Figure 3.

Icariin restored the Hcy-reduced acetylation and phosphorylation of tubulin in cultured neuronal cells. (A–F): Primary cortical neuronal cultures were treated with 200 µM Hcy for 48 h. For the co-treatment, cells were pretreated with icariin for 1 h, before incubating with Hcy for 48 h. The treated cells were subjected for western blot analyses and quantified for the detection of Ac- and Tyr-tubulin with anti-Ac-α-tubulin and anti-tyr-antibodies. (G–H) Western blot analysis for the detection of p-tau and total tau with anti-p-tau and anti-tau antibodies. Cells were treated 200αM Hcy alone for 2 h. For the co-treatment, cells were pretreated with icariin for 1 h, followed by co-incubation with Hcy for another 2 h. α-tubulin was used as internal control. Data represent mean ± SE from at least three independent experiments and was quantified using densitometry. *: p < 0.05 compared to control. ** and #: p < 0.05 compared to cultures treated with 200 μM Hcy. “+” and “-”: with and without the compound.