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. 2016 Oct 14;21(10):1364. doi: 10.3390/molecules21101364

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© 2016 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Solasonine inhibits the Hh signaling pathway by targeting Gli (A) Solasonine dose-dependently inhibits the Gli-luciferase activity provoked by SAG. The results are expressed as mean ± s.d.; (B). Expression of Sufu in NIH-3T3 cells infected with lentivirus carrying Sufu shRNA or shRNA control. The cells infected with various types of lentivirus were collected for Western blot analyses. Immunoblotting of GAPDH served as a loading control; (C,D) Solasonine suppressed the mRNA expression of Gli1 (C) and ptch1 (D) caused by limiting Sufu expression. The results are expressed as mean ± s.d.; (E) Ectopic expression of Gli1 in light II cells as examined by western blot analysis; (F) Solasonine suppressed the Gli-luciferase activity caused by exogenous expression Gli1 in a dose-dependent manner. Data are expressed as mean ± s.d. (n = 3); (G) Ectopic expression of Gli2 in light II cells as examined by western blot analysis; (H) Solasonine suppressed the Gli-luciferase activity caused by exogenous expression Gli2 in a dose-dependent manner. Data are expressed as mean ± s.d. (n = 3). Statistical differences were analyzed by the two-tailed Student′s t test and p < 0.05 was considered as significant (* p < 0.05; ** p < 0.01).

Solasonine inhibits the Hh signaling pathway by targeting Gli (A) Solasonine dose-dependently inhibits the Gli-luciferase activity provoked by SAG. The results are expressed as mean ± s.d.; (B). Expression of Sufu in NIH-3T3 cells infected with lentivirus carrying Sufu shRNA or shRNA control. The cells infected with various types of lentivirus were collected for Western blot analyses. Immunoblotting of GAPDH served as a loading control; (C,D) Solasonine suppressed the mRNA expression of Gli1 (C) and ptch1 (D) caused by limiting Sufu expression. The results are expressed as mean ± s.d.; (E) Ectopic expression of Gli1 in light II cells as examined by western blot analysis; (F) Solasonine suppressed the Gli-luciferase activity caused by exogenous expression Gli1 in a dose-dependent manner. Data are expressed as mean ± s.d. (n = 3); (G) Ectopic expression of Gli2 in light II cells as examined by western blot analysis; (H) Solasonine suppressed the Gli-luciferase activity caused by exogenous expression Gli2 in a dose-dependent manner. Data are expressed as mean ± s.d. (n = 3). Statistical differences were analyzed by the two-tailed Student′s t test and p < 0.05 was considered as significant (* p < 0.05; ** p < 0.01).