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. 2016 Oct 14;21(10):1364. doi: 10.3390/molecules21101364

Figure 5.

Figure 5

Solasonine inhibits the Hh pathway activity provoked by ectopic expressions of distinct Smo plasmids. (A) Ectopic expression of SmoD473H in light II cells as examined by western blot analysis; (B) Solasonine suppressed the Gli-luciferase activity provoked by exogenous SmoD473H. Light II cells transfected with GFP and SmoD473H plasmids were exposed to various treatments as indicated for 36 h. Data are expressed as mean ± s.d. (n = 3); (C) Ectopic expression of SmoW539L in light II cells as examined by western blot analysis; (D) Solasonine suppressed the Gli-luciferase activity provoked by artificially forced expression of SmoW539L. Light II cells transfected with GFP and SmoW539L plasmids were subjected to various treatments as indicated for 36 h. Data are expressed as mean ± s.d. (n = 3); (E) Ectopic expression of Smo wild type in light II cells as examined by western blot analysis; (F) Solasonine blocked the Gli-luciferase activity stimulated by artificially forced expression of Smo wild type. Light II cells transfected with GFP and Smo wild type plasmids were subjected to various treatments as indicated for 36 h. Data are expressed as mean ± s.d. (n = 3). Statistical differences were analyzed by the two-tailed Student′s t test and p < 0.05 was considered as significant (* p < 0.05; ** p < 0.01).