REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-nc82	DSHB	Cat# nc82; RRID: AB_2314866
Rat monoclonal DYKDDDDK Epitope Tag Antibody (L5) (bind to Flag)	Novus Biologicals	Cat# NBP1–06712; RRID: AB_1625981
Rabbit monoclonal anti-HA-Tag	Cell Signal Technologies	Cat#3724S; RRID: AB_1549585
Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405)	Abcam	Cat#ab175658
Donkey Anti-Rat IgG H&L (Alexa Fluor® 647)	Abcam	Cat#ab150155
Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488)	Abcam	Cat#ab150073
Mouse anti V5-Tag:DyLight®550	Bio-Rad (Formerly AbD Serotec)	Cat#MCA1360D5 50GA
Experimental Models: Organisms/Strains
P{20XUAS-IVS-GCaMP6f}attP40	Bloomington Drosophila Stock Center	BDSC: 42747
HS-GAL4: P{GMR27B03-GAL4}attP2	Bloomington Drosophila Stock Center	BDSC: 49211
VS-GAL4: P{GMRR78F01-GAL4}attP2	Bloomington Drosophila Stock Center	BDSC: 49620
T4/T5-GAL4: P{GMR42F06-GAL4}attP2	Bloomington Drosophila Stock Center	BDSC: 41253
MCFO1: P{hs-FLPG5.PEST}attP3;PBac{10xUAS(FRT.stop)m yr::smGdP-HA}VK00005 P{10xUAS(FRT.stop)myr::smGdP-V5-THS-10xUAS(FRT.stop)myr::smGdPFLAG}su(Hw)attP1	Bloomington Drosophila Stock Center	BDSC:64085
Software and Algorithms
Python 2.7	Python	https://www.python.org
PsychoPy v1.82	PsychoPy	http://www.psychopy.org/
Fiji	Fiji	https://fiji.sc/
custom Python code	This study	This study