Figure 4.

The thermal imaging camera recorded the temperature fluctuations of the 96-well plate containing lung cancer cells incubated with AuNRs. (a) The recorded temperature changes of the target well under PW illumination, corresponding to the red circle in (b), for 5 min for the Au10s (blue), Au25s (red), Au40s (green), and Au50s (yellow) with the media not replaced, and (b) A single frame taken with the thermal camera during the PW-PPTT of lung cancer cells containing AuNRs. The red circle indicates the well under PW laser exposure. The data demonstrates that PW lasers do not induce a bulk temperature change regardless of AuNR size. The observed temperature fluctuations in (a) are a result of minute room-temperature changes. Note: Due to the limitations of infrared cameras, the displayed temperature measurements represent the temperature of the plastic bottom of the 96-well plate.