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. 2018 Oct 28;19(11):3369. doi: 10.3390/ijms19113369

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunostaining of CYP2E1 in SH-SY5Y cells after β-NF and EtOH treatment. Representative immunofluorescence images of each experimental condition. Column 1 represents mitochondrial stain (red), column 2 represents ER-Green Fluorescent Protein (GFP) stain (green), and column 3 reports the CYP2E1 staining (blue). Column 4 represents the merge between CYP2E1 and mitochondria channel, column 5 represents the merge between CYP2E1 and ER-GFP, and column 6 represents the merge of the three channels. R Values: Pearson’s correlation coefficient. N/A: Not determined. Scale bars: 20 µm (control), 40 µm (β-NF and EtOH).