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. 2018 Nov 7;19(11):3506. doi: 10.3390/ijms19113506

Table 3.

The gradient conditions for hydrophilic separation of polar metabolites.

Time (min) A (v%) B (v%)
0 95 5
1 95 5
7 50 50
9 50 50
9.1 95 5
13 95 5