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. 2018 Nov 14;19(11):3591. doi: 10.3390/ijms19113591

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Gγ3 and Gγ3ΔCys were enriched in the plasma membrane fraction of the wild-type (WT) and Minute (Mi) flowers. First 10 μg of both the crude microsomal fraction protein and plasma membrane fraction protein from the WT and Mi were analyzed by western blot using anti-OsPIP1s, anti-Gα, anti-Gβ, and anti-Gγ3 domain antibodies. OsPIP1s is an aquaporin, which is a plasma membrane marker. OsPIP1s (25 kDa), Gα (45 kDa), Gβ (4 3kDa), Gγ3 (32 kDa), and Gγ3ΔCys (20 kDa) are indicated by arrows. Non-specific bands are indicated by arrow heads.