Table 1.

LC-MS/MS analysis of Gγ3 fragments in in the plasma membrane of the wild-type (WT) and Minute (Mi) flowers.

(A) Gγ3 fragments in the plasma membrane fraction of the WT flower
Fragments	Observed	Mr(expt)	Mr(calc)	Expected	Peptide
1	379.2251	1134.6536	1134.6509	0.00078	R.LQLAVDALHR.E
2	714.7006	2141.08	2141.0753	0.00000034	R.EIGFLEGEINSIEGIHAASR.C
3	482.7414	963.4682	963.4662	0.007	R.EVDEFIGR.T
(B) Gγ3 fragments in the plasma membrane fraction of the Mi flower
Fragment	Observed	Mr(expt)	Mr(calc)	Expected	Peptide
2	714.7014	2141.0824	2141.0753	0.0000024	R.EIGFLEGEINSIEGIHAASR.C
3	482.7408	963.4671	963.4662	0.0077	R.EVDEFIGR.T
4-1	667.8468	1333.6791	1333.6765	0.00015	R.TPDPFITISSEK.R
(C) Gγ3 fragments in the immunoprecipitation products using the plasma membrane fraction of WT flower
Fragments	Observed	Mr(expt)	Mr(calc)	Expected	Peptide
1	568.3348	1134.6551	1134.6509	5.40 × 10−7	R.LQLAVDALHR.E
3	482.7417	963.4688	963.4662	0.00062	R.EVDEFIGR.T
4-2	497.6015	1489.7827	1489.7776	2.50 × 10−5	R.TPDPFITISSEKR.S
(D) Gγ3 fragments in the immunoprecipitation products using the plasma membrane fraction of Mi flower
Fragments	Observed	Mr(expt)	Mr(calc)	Expected	Peptide
1	568.3354	1134.6562	1134.6509	8.90 × 10−7	R.LQLAVDALHR.E
2	714.7017	2141.0832	2141.0753	8.60 × 10−7	R.EIGFLEGEINSIEGIHAASR.C
3	482.7427	963.4709	963.4662	0.00072	R.EVDEFIGR.T
4-1	667.8485	1333.6825	1333.6765	7.70 × 10−6	R.TPDPFITISSEK.R

Forty micrograms of each protein of the plasma membrane fraction of the wild-type (WT) and Minute (Mi) (A,B) and 5 μL of each eluate in the immunoprecipitation experiment of WT and Mi (C,D) were used for LC-MS/MS. Fragments of the trypsin-digested Gγ3 candidates (p < 0.05) are shown. The fragment numbers correspond to Figure 4A. Mr(expt) and Mr(calc) correspond to the theoretical molecular mass and the molecular mass that was calculated from the observed molecular mass, respectively. The scores from the Mascot search were 91 (A), 90 (B), 164 (C), and 248 (D).