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. 2018 Oct 23;19(11):3291. doi: 10.3390/ijms19113291

Figure 2.

Figure 2

Semiquantitative PCR and qRT-PCR (quantitative real-time PCR) data of the transcript levels of the Ghrack1 and Ghuhrf1 genes in different tissues of Gossypium hirsutum. (a) Semiquantitative PCR survey of various cotton tissues for the detection of Ghrack1 and Ghuhrf1 transcripts. Ghsad1 was used as a control. (b,c) Relative transcript abundance of two Gossypium hirsutum genes, that is, Ghrack1 (a) and Ghuhrf1 (c), detected in various cotton tissues by qRT-PCR. The data represent the relative expression of the Ghrack1 and Ghuhrf1 transcripts ± SD of three biological replicates in each tissue (n = 3). Asterisks and double asterisks indicate significant deviations from the root at p < 0.05 and p < 0.01, respectively, using the Student’s t-test for comparisons between the root and other tissues separately for both genes. R (root), L (leaf), A (anther), S (stigma), 0 dpa (0 dpa fiber), 5 dpa (5 dpa fiber), 7 dpa (7 dpa fiber), 14 dpa (14 dpa fiber), 21 dpa (21 dpa fiber), 26 dpa (26 dpa fiber), and 28 dpa (28 dpa fiber).