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. 2018 Oct 23;19(11):3291. doi: 10.3390/ijms19113291

Figure 4.

Figure 4

Transient expression of the gus gene and gfp gene in tobacco leaves, using the epidermis infection method. (a) Schematic diagram of the promoter-reporter gene constructs GFP::GUS, 35S::GUS, GhZUf::GUS, and GFP::GhZU::GUS, used for the transient assay in the N. benthamiana leaf using the pCambia1305 vector. (b) Schematic diagram of the promoter-reporter gene constructs GFP::GUS, 35S::GFP, GhZUr::GFP, and GFP::GhZU::GUS, used for the transient assay in the N. benthamiana leaf using the pCambia1305 vector. Below each construct, a representative assay of transient gus expression detected histochemically, transient gfp expression detected based on fluorescence imaging via the Agrobacterium infiltration assay in N. benthamiana leaf, and the respective promoter, no promoter, 35S promoter, Ghuhrf1 promoter (GhZUf), and Ghrack1 promoter (GhZUr) activities are shown.