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Identification of interactors of TBC1D21 through co-immunoprecipitation (co-IP) and nano LC–MS/MS. (A,B) Tandem mass spectrometry (MS/MS) spectrum of the tryptic peptides of Rap1: SALTVQFVQGIFVEK and NGQGFALVYSITAQSTFNDLQDLR. (C) Immunoblotting (IB) of NTERA-2 cl.D1 (NT2D1) cell lysates with the anti-Rap1 and anti-β-actin antibodies.
