Figure 3.

Effect of silver nanoparticles on S. aureus and P. aeruginosa, E. coli, and S. uberis from mastitis-infected goats. (A) Cell viability of S. aureus and P. aeruginosa treated with AgNPs. (B) Cell viability of E. coli, and S. uberis treated with AgNPs. Bacterials cells were incubated with various concentrations of AgNPs. Bacterial survival was determined at 24 h by a CFU (colony forming unit) count assay. The experiment was performed with various controls, including a positive control (AgNPs and NB, without inoculum) and a negative control (NB and inoculum, without AgNPs). The results are expressed as the means ± SD of three separate experiments, each of which contained three replicates. Treated groups showed statistically significant differences from the control group by Student’s t test (p < 0.05).