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. 2018 Nov 13;19(11):3578. doi: 10.3390/ijms19113578

Figure 5.

Figure 5

(A) Comparison of PKC β immunofluorescence (green) between representative samples from a db/db mouse treated with vehicle, a db/db mouse treated with bosentan, and a non-diabetic mouse (db/+). Nuclei were labeled with Hoechst (blue). Scale bar: 20 µm. GCL: Ganglion cell layer; IPL: Inner plexiform layer; INL: Inner cell layer; OPL: Outer plexiform layer; ONL: Outer nuclear layer; (B) Quantification of PKC β immunofluorescence. A.U.: Arbitrary units. Data are expressed as mean ± SD. * p < 0.05 in comparison with the other groups; (C) TNF-α (red) immunofluorescence retinal images from a representative db/db mouse treated with vehicle, a db/db mouse treated with bosentan and a non-diabetic (db/+) mouse. Blood vessels were immunostained with collagen IV (green). Magnification at 100× shows the expression of TNF-α in retinal vessels in a representative diabetic mouse but not in a representative mouse from the other interventional groups.