Table 1.
Gene | Primer Sequence | Product Size (bp) | GeneBank Accession Nr. |
---|---|---|---|
Gapdh | S 5′-ACC CAG AAG ACT GTG GAT GG-3′ A 5′-CAC ATT GGG GGT AGG AAC AC-3′ |
171 | XM_132897 |
18s rRNA | S 5′-GTA ACC CGT TGA ACC CCA TT-3′ A 5′-CCA TCC AAT CGG TAG TAG CG-3′ |
151 | X00686 |
Trap | S 5′-GCG ACC ATT GTT AGC CAC ATA CG-3′ A 5′-CGT TGA TGT CGC ACA GAG GGA T-3′ |
144 | NM_007388.2 |
CalcR | S 5′-TGG TGC GGC GGG ATC CTA TAA GT-3′ A 5′-AGC GTA GGC GTT GCT CGT CG-3′ |
150 | NM_001042725 |
Ctsk | S 5′-AGC AGA ACG GAG GCA TTG ACT C-3′ A 5′-TTT AGC TGC CTT TGC CGT GGC-3′ |
92 | NM_007802.3 |
H+ATPase | S 5′-ACG GTG ATG TCA CAG CAG ACG T-3′ A 5′-CCT CTG GAT AGA GCC TGC CGC A-3′ |
153 | NM_175406.3 |
Mmp9 | S 5′-GCT GAC TAC GAT AAG GAC GGC A-3′ A 5′-GCG GCC CTC AAA GAT GAA CGG-3′ |
114 | NM_013599.2 |
All the primers were of mouse source. S: sense. A: antisense.