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. 2018 Oct 25;19(11):3319. doi: 10.3390/ijms19113319

Table 1.

Sequence of the specific primers used in the real time RT-PCR analysis of the reference and target genes for the in vitro study.

Gene Primer Sequence Product Size (bp) GeneBank
Accession Nr.
Gapdh S 5′-ACC CAG AAG ACT GTG GAT GG-3′
A 5′-CAC ATT GGG GGT AGG AAC AC-3′
171 XM_132897
18s rRNA S 5′-GTA ACC CGT TGA ACC CCA TT-3′
A 5′-CCA TCC AAT CGG TAG TAG CG-3′
151 X00686
Trap S 5′-GCG ACC ATT GTT AGC CAC ATA CG-3′
A 5′-CGT TGA TGT CGC ACA GAG GGA T-3′
144 NM_007388.2
CalcR S 5′-TGG TGC GGC GGG ATC CTA TAA GT-3′
A 5′-AGC GTA GGC GTT GCT CGT CG-3′
150 NM_001042725
Ctsk S 5′-AGC AGA ACG GAG GCA TTG ACT C-3′
A 5′-TTT AGC TGC CTT TGC CGT GGC-3′
92 NM_007802.3
H+ATPase S 5′-ACG GTG ATG TCA CAG CAG ACG T-3′
A 5′-CCT CTG GAT AGA GCC TGC CGC A-3′
153 NM_175406.3
Mmp9 S 5′-GCT GAC TAC GAT AAG GAC GGC A-3′
A 5′-GCG GCC CTC AAA GAT GAA CGG-3′
114 NM_013599.2

All the primers were of mouse source. S: sense. A: antisense.