Table 2.
Gene | Primer Sequence | Primer Source | Product Size (bp) | GeneBank Accession Nr. |
---|---|---|---|---|
GAPDH | S 5′- TGC ACC ACC AAC TGC TTA GC-3′ A 5′- GGC ATG GAC TGT GGT CAT GAG-3′ |
Human | 87 | M33197 |
18s rRNA | S 5’-GTA ACC CGT TGA ACC CCA TT-3’ A 5’-CCA TCC AAT CGG TAG TAG CG-3’ |
Mouse | 151 | X00686 |
IL-10 | S 5′- CCT TTG GCA GGG TGA AGA CT-3′ A 5′- ATG GCT GGA CTC TGG TTC TC-3 |
Rabbit | 175 | NM_001082045 |
Tnf-α | S 5′- TCC GTG AAA ACA GAG CAG AA-3′ A 5′- GAG CAG AGG TTC GGT GAT GT-3′ |
Rabbit | 160 | NM_001082263 |
Opg | S 5′-TCA TCC AAG ATA TTG ACC TCT GTG A-′3 A 5′-GGGGAGCTGCTCACTTGATT-3′ |
Rabbit | 170 | XM_002710603.2 |
RANKL | S 5’- CAG AGC GCA GAT GGA TCC TAA-3′ A 5′- TCC TTT TGC ACA GCT CCT TGA-3′ |
Human | 180 | NM_003701.3 |
Trap | S 5′- CCT GGG CGA CAA CTT TTA CT-3′ A 5′- TTG GAG ACC TTG GAA TAG GC-3′ |
Rabbit | 180 | NM_001081988 |
Calc-R | S 5′- CAA ATG ACA CCC ATC CAA CA-3′ A 5′- ACA TCC ATC CAT CCC AGG TC-3′ |
Rabbit | 162 | NM_001082375.2 |
Ctsk | S 5′-GAC ACC CAG TGG GAG CTA TG-3′ A 5′-TCT TCA CTG GTC ATG TCC CC-3′ |
Rabbit | 188 | NM_001082641.1 |
H+ATPase | S 5′ -CCG AAA CCT CCT GAA GAA AA-3′ A 5′- ATA GCC GTG GTG CTG AAG TC-3′ |
Rabbit | 165 | NM_000600.3 |
Mmp9 | S 5′-CAA GGA TGG GAG GTA CTG GC-3′ A 5′-TGT GTA CAC CCA CAC TTG GC-3′ |
Rabbit | 172 | NM_001082203.1 |
S: sense. A: antisense.