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. 2018 Oct 25;19(11):3319. doi: 10.3390/ijms19113319

Table 2.

Sequence of the specific primers used in the real time RT-PCR analysis of the reference and target genes for the in vivo study.

Gene Primer Sequence Primer Source Product Size (bp) GeneBank
Accession Nr.
GAPDH S 5′- TGC ACC ACC AAC TGC TTA GC-3′
A 5′- GGC ATG GAC TGT GGT CAT GAG-3′
Human 87 M33197
18s rRNA S 5’-GTA ACC CGT TGA ACC CCA TT-3’
A 5’-CCA TCC AAT CGG TAG TAG CG-3’
Mouse 151 X00686
IL-10 S 5′- CCT TTG GCA GGG TGA AGA CT-3′
A 5′- ATG GCT GGA CTC TGG TTC TC-3
Rabbit 175 NM_001082045
Tnf-α S 5′- TCC GTG AAA ACA GAG CAG AA-3′
A 5′- GAG CAG AGG TTC GGT GAT GT-3′
Rabbit 160 NM_001082263
Opg S 5′-TCA TCC AAG ATA TTG ACC TCT GTG A-′3
A 5′-GGGGAGCTGCTCACTTGATT-3′
Rabbit 170 XM_002710603.2
RANKL S 5’- CAG AGC GCA GAT GGA TCC TAA-3′
A 5′- TCC TTT TGC ACA GCT CCT TGA-3′
Human 180 NM_003701.3
Trap S 5′- CCT GGG CGA CAA CTT TTA CT-3′
A 5′- TTG GAG ACC TTG GAA TAG GC-3′
Rabbit 180 NM_001081988
Calc-R S 5′- CAA ATG ACA CCC ATC CAA CA-3′
A 5′- ACA TCC ATC CAT CCC AGG TC-3′
Rabbit 162 NM_001082375.2
Ctsk S 5′-GAC ACC CAG TGG GAG CTA TG-3′
A 5′-TCT TCA CTG GTC ATG TCC CC-3′
Rabbit 188 NM_001082641.1
H+ATPase S 5′ -CCG AAA CCT CCT GAA GAA AA-3′
A 5′- ATA GCC GTG GTG CTG AAG TC-3′
Rabbit 165 NM_000600.3
Mmp9 S 5′-CAA GGA TGG GAG GTA CTG GC-3′
A 5′-TGT GTA CAC CCA CAC TTG GC-3′
Rabbit 172 NM_001082203.1

S: sense. A: antisense.