Figure 6.

Effect of combinatorial treatment with Sorafenib and tumor-treating fields (TTFields) on the invasiveness and migration of glioblastoma cells. (A) tumor cell migration was assessed using a Transwell chamber assay. * p < 0.05; ** p < 0.01, bar = 500 µm; (B) tumor cell invasion was assessed using a Matrigel invasion assay. * p < 0.05; ** p < 0.01, bar = 500 µm; (C) cell lysates prepared from sorafenib-, TTFields-, and sorafenib plus TTFields-treated cells were used in Western blotting using antibodies against vimentin and fibronectin; Band intensities were quantified and normalized to actin intensities (n = 3, mean ± SD). (D) tube formation assay using 2H11 cells subjected to the indicated treatments; (E) 3D colony cultures of 2H11 cells treated as indicated. ** p < 0.01.