Figure 4.

Effects of LY294002 (PI 3-kinase inhibitor) and PD98059 (mitogen activated protein kinase kinase inhibitor) on HDM-allergen stimulation of ROS generation in human airway epithelial cell line Calu-3. Data are shown as mean ± standard error values (n = 8). * p < 0.001 vs vehicle controls; ** p < 0.001 vs allergen-activated cells (1-way ANOVA with post-hoc testing by Student Newman Keuls procedure). The ROS production was quantified in cells loaded with dihydrorhodamine 123 as described in [78,103].