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. 2018 Nov 14;19(11):3592. doi: 10.3390/ijms19113592

Figure 4.

Figure 4

CXCR4 and ACKR3 follow different internalization pathways after CXCL12 stimulation. (A) CHO-CXCR4, (B) CHO-ACKR3, (C) MDA-MB-231-CXCR4, (D) MDA-MB-231-ACKR3 and (E) CHO-CXCR4-ACKR3 cells were treated with 10–50 nM CXCL12 for 15–30 min, then washed and incubated with chemokine-free media for up to 2 h to assess receptor recycling. Cells were labelled with CXCR4-PE and/or ACKR3-APC antibody and receptor’s mean fluorescence intensity (MFI) was measured using flow cytometry. Data represents the mean ± SEM of three independent experiments and statistical significance was calculated using a one way ANOVA (ns: not significant, * p < 0.05, ** p < 0.01, *** p < 0.001).