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. 2018 Nov 14;19(11):3592. doi: 10.3390/ijms19113592

Figure 6.

Figure 6

CXCR4 and ACKR3 follow different internalization pathways after VUF11207 stimulation. (A) CHO-CXCR4, (B) CHO-ACKR3 and (C) CHO-CXCR4-ACKR3 cells were stimulated with 1 nM VUF11207 for 30 min and then washed and incubated with agonist-free media for up to 2 h to assess receptor recycling. Cells were then labelled and receptor expression was measured using flow cytometry. Data represents the mean ± SEM of three independent experiments and statistical significance was calculated using a one way ANOVA (ns: not significant, * p < 0.05, ** p < 0.01, *** p < 0.001). (D) CXCL12 and VUF11207 have a different effect in ACKR3 transcription. CHO-ACKR3 cells were stimulated with 1 nM VUF11207 or 10 nM CXCL12 and recycling was assessed as described above. RNA was then extracted at each time point and ACKR3 expression was assessed using qPCR and normalized to GAPDH. Data represents the mean ± SEM of three independent experiments and statistical significance was calculated using a one-way ANOVA (* p < 0.05, *** p < 0.001).