Table 4.
Results of secondary structure content analysis using FT-IR and parameters of thermograms recorded by DSC of glutelin fraction.
| Sample | Secondary Structures (%) | Thermostability | |||||||
|---|---|---|---|---|---|---|---|---|---|
| α-Helix | β-Sheet | β-Turn | Random Coil | To (°C) | Te (°C) | Tp (°C) | ∆H (J/g) | To (°C) | |
| NR-glutelin | 19.59 | 44.51 | 11.65 | 24.25 | 50.07 | 106.93 | 90.48 | 14.91 | 50.07 |
| SR25-glutelin | 21.15 | 42.57 | 12.35 | 23.92 | 45.57 | 127.86 | 92.04 | 14.88 | 45.57 |
| SR50-glutelin | 21.73 | 39.29 | 14.60 | 24.36 | 47.10 | 132.38 | 85.39 | 28.81 | 47.10 |
| SR75-glutelin | 33.91 | 39.64 | 9.64 | 24.45 | 51.80 | 141.79 | 88.26 | 29.19 | 51.80 |
| SR100-glutelin | 21.64 | 39.44 | 14.47 | 16.81 | 49.23 | 127.44 | 86.58 | 18.62 | 49.23 |
To and Te are the initial and final temperatures of endothermic transition. Tp is the temperature at peak maximum and ∆H is the enthalpy of denaturation.