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KM treatment prevents apoptosis-related morphological changes in the nuclei of AGE-treated GMCs. Detection of apoptotic morphology using Hoechst 33258 stain in (A) control group cells (B) cells treated with AGEs (160 μg/mL) for 24 h, and (C–E) cells pre-treated with 10, 20, or 35 μM KM and then stimulated with AGEs (160 μg/mL) for 24 h. The photographs were taken directly from culture plates with an inverted fluorescence microscope (magnification 100×).
