Table 1.

Details of the most important metabolic values in PLS-DA classification. The p-Values of each time comparison are reported in the table, as well as not significant (ns) p-values. T1 vs. T2: First Trimester vs. Second Trimester; T2 vs. T3: Second Trimester vs. Third Trimester; T1 vs. T3: First Trimester vs. Third Trimester; T1 vs. post-partum (P-P): First Trimester vs. post-partum; T2 vs. P-P: Second Trimester vs. P-P; T3 vs. P-P: Third Trimester vs. P-P; the data were obtained by unpaired t test with Welch’s correction. VIP: Variable Importance of Projection; Sa: Sphinganine; Sa1P: Sphinganine-1-Phosphate; C16Cer: Ceramide (d18:1/16:0); C24dHCer: Dihydroceramide (d18:1/24:1): C0: Free Carnitine; So1P: Sphingosine-1-Phosphate; C16GlcCer: Glucosylceramide (d18:1/16:0); C24: Tetracosanoylcarnitine; C2: Acetylcarnitine; C18Cer: Ceramide (d18:1/18:0); C18:1: Octadecenoylcarnitine.

Metabolite	VIP Value	Kruskal–Wallis p-Value	T1 vs. T2	T2 vs. T3	T1 vs. T3	T1 vs. P-P	T2 vs. P-P	T3 vs. P-P
So	2.29	<0.0001	0.0001	ns	0.05	0.05	ns	ns
Sa1P	1.80	<0.0001	0.0001	0.01	ns	0.01	ns	ns
C16Cer	1.60	<0.01	0.05	ns	0.05	ns	ns	ns
C24dHCer	1.57	<0.001	0.01	ns	0.01	0.05	ns	ns
C0	1.41	<0.0001	ns	ns	ns	0.05	0.0001	0.0001
So1P	1.41	<0.01	0.0001	ns	ns	ns	0.05	ns
C16GlcCer	1.32	<0.0001	ns	ns	0.05	ns	0.01	0.0001
C24	1.32	<0.001	ns	ns	0.01	0.01	ns	ns
C2	1.32	<0.0001	ns	ns	ns	0.01	0.0001	0.0001
GLY	1.27	<0.001	ns	ns	ns	0.01	0.0001	ns
ALA	1.25	<0.01	ns	ns	0.05	0.01	ns	ns
C18Cer	1.23	<0.01	ns	ns	0.01	ns	ns	ns
GLNLYS	1.21	<0.001	ns	ns	ns	0.05	0.0001	0.05
TYR	1.09	<0.001	ns	ns	ns	0.05	0.01	0.05
PRO	1.08	<0.01	ns	ns	ns	0.01	0.05	ns
Sa	1.02	<0.0001	0.01	0.01	ns	0.0001	ns	0.0001
C18:1	1.00	<0.01	ns	ns	ns	ns	0.01	ns