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. 2018 Oct 30;19(11):3394. doi: 10.3390/ijms19113394

Figure 3.

Figure 3

Competition assays between HDL cholesterol and low-density (LDL) cholesterol internalization in HMEC-1. Cultured cells were coincubated with a constant rHDL cholesterol concentration (50 mg/dL) and increasing concentrations (20 to 2000 mg/dL) of cholesterol associated with LDL isolated from human plasma. rHDL were labeled with 25-NBD-cholesterol, and internalization was monitored by flow cytometry. Data correspond to mean ± SD of three independent experiments. * p < 0.05 vs. HDL 50 mg/dL and no LDL.