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. 2018 Oct 26;19(11):3345. doi: 10.3390/ijms19113345

Figure 3.

Figure 3

rhsfCR-1 attenuated the sphere formation ability of miPS-LLCcm cells. (A) The number of spheres were counted after the treatment with various concentrations of rhsfCR-1 for 1 week under non-adherent condition. Student t-test was conducted to analyze the significance (* p < 0.05, *** p < 0.001); (B) spheres in non-adherent cultures in serum free miPS medium supplemented with ITS-x. Spheres were photographed after the treatment with/without rhsfCR-1 (1 µg/mL) for 1 week using sphere formation assay; (C) extreme limiting dilution assay assessment of the limiting dilution sphere forming potential showed significantly reduced sphere formation of miPS-LLCcm cells in the presence of rhsfCR-1 at a high cell density per well in 96-well low attachment plates. (See Materials and Methods, Section 4).