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. 2018 Nov 10;19(11):3546. doi: 10.3390/ijms19113546

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Phospho-p70S6K inhibitor (PF-4708671) inhibited the migratory and invasion activities of SCC-4 and SCC-25 cells. (A) Wound-healing assay revealed that SCC-4 and SCC-25 cells had shorter migration distances after PF-4708671 treatment. * p < 0.05, ** p < 0.01, and *** p < 0.001 indicate the statistical significance of the differences at one point in time between cells with and without treatment. (B) After incubating for 24 h (SCC-4) or 48 h (SCC-25) in transwell chambers, the area of migratory or invasive cells was markedly decreased in PF-4708671-treated cells compared with cells without treatment. * p < 0.05 indicates the statistical significance of differences between cells with and without treatment (red * for SCC-4 and blue * for SCC-25).