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. 2018 Oct 26;19(11):3341. doi: 10.3390/ijms19113341

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Human periosteum-derived cell (hPDC) viability within PEG-VS hydrogels made with different concentrations of macromer, different protease sensitive cross-linkers (R or F), and with or without the cell binding motif RGD or scrambled RDG after 1 week (A) and 4 weeks (B) of in vitro culture in GM. Representative images of Live/Dead staining with calcein AM (green, live cells) and ethidium homodimer-1 (red, dead cells). Confocal images are depicted as the maximum intensity of a 500 μm z-stack that was acquired using a 10× objective (scale bars = 200 μm). (C) IMARIS software based quantification of viability percentages of cells encapsulated in polyethylene glycol (PEG) hydrogels (n = 1 independent sample; values depicted are the averages of two different measured areas within one sample).

Human periosteum-derived cell (hPDC) viability within PEG-VS hydrogels made with different concentrations of macromer, different protease sensitive cross-linkers (R or F), and with or without the cell binding motif RGD or scrambled RDG after 1 week (A) and 4 weeks (B) of in vitro culture in GM. Representative images of Live/Dead staining with calcein AM (green, live cells) and ethidium homodimer-1 (red, dead cells). Confocal images are depicted as the maximum intensity of a 500 μm z-stack that was acquired using a 10× objective (scale bars = 200 μm). (C) IMARIS software based quantification of viability percentages of cells encapsulated in polyethylene glycol (PEG) hydrogels (n = 1 independent sample; values depicted are the averages of two different measured areas within one sample).