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. 2018 Oct 26;19(11):3341. doi: 10.3390/ijms19113341

Figure 6.

Figure 6

ATDC viability within protease sensitive 6.5% (w/v) PEG-VS hydrogels, with different cross-linkers (F or R) as well as with and without RGD, cultured over time. Constructs were examined at 0 weeks (24 h in MM) and 1 and 4 weeks in the 4C chondrogenic differentiation medium. 6.5R0 = R cross-linker + 0 µM RGD; 6.5RR = R cross-linker + 150 µM RGD; 6.5F0 = F cross-linker + 0 µM RGD; 6.5FR = F cross-linker + 150 µM RGD. (A) IMARIS software based quantification of viability percentages of ATDC5 cells encapsulated in PEG hydrogels (n = 1; values depicted are the averages of two different measured areas within one sample). (B) Representative confocal images of Live/Dead staining shown as the maximum intensity of a 500 µm z-stack where live cells are green and dead cells are red (scale bar = 200 µm).