Table 3.
Proteins inconsistently regulated in embryos of germinated rice seeds in response to BR and GA deficiency by iTRAQ analysis.
| Gene ID | NIP (BRZ/Mock) | sd1/NIP | Protein | Unique | Sequence | Description |
|---|---|---|---|---|---|---|
| Fold Change a | Fold Change b | Score c | Peptide d | Coverage (%) e | ||
| Os01g0210500 | 0.762 | 1.366 | 1133 | 6 | 45 | Similar to SOUL-like protein |
| Os01g0233000 | 1.237 | 0.611 | 391 | 5 | 34.3 | DREPP plasma membrane polypeptide family protein |
| Os01g0294700 | 1.239 | 0.694 | 707 | 8 | 33.7 | Haem peroxidase, plant/fungal/bacterial family protein |
| Os02g0209300 | 0.69 | 1.333 | 78 | 1 | 13.7 | Hypothetical conserved gene |
| Os03g0341100 | 1.234 | 0.767 | 506 | 4 | 28.3 | Similar to 60S ribosomal protein L18 |
| Os03g0379100 | 1.252 | 0.811 | 176 | 3 | 6.9 | Protein of unknown function DUF248 |
| Os03g0700400 | 1.297 | 0.408 | 1056 | 3 | 37.3 | Similar to LOX4 |
| Os03g0842900 | 0.768 | 1.28 | 1047 | 13 | 43.9 | Similar to steroleosin-B |
| Os04g0165300 | 0.825 | 1.271 | 74 | 2 | 8.9 | Conserved hypothetical protein |
| Os04g0390800 | 0.818 | 1.244 | 2520 | 16 | 54.8 | NAD(P)-binding domain containing protein |
| Os04g0497200 | 1.275 | 0.735 | 169 | 4 | 9.3 | Cellulase precursor |
| Os04g0546500 | 0.807 | 1.31 | 836 | 2 | 13.5 | Similar to oleosin |
| Os05g0268500 | 0.451 | 1.478 | 131 | 5 | 12 | Similar to serine carboxypeptidase 2 |
| Os05g0474400 | 1.253 | 0.775 | 197 | 2 | 10.9 | Prenylated rab acceptor PRA1 family protein |
| Os06g0675700 | 0.463 | 1.229 | 831 | 4 | 18 | Similar to high pI alpha-glucosidase |
| Os11g0582400 | 0.626 | 1.385 | 702 | 2 | 5.1 | Similar to embryo-specific protein |
a NIP (BRZ/Mock) fold change indicates fold change of protein abundance between BRZ treated samples and the mock-treated control. b sd1/NIP fold change indicates fold change of protein abundance between sd1 mutant and the NIP control. c Protein score is based on combined mass spectroscopy (MS) and tandem mass spectroscopy (MS/MS) spectra. The proteins that had a statistically significant (p < 0.05) protein score of 70 or more were considered successfully identified. d Unique peptide indicates the number of identified unique peptides for confident protein identification. e The protein with a sequence coverage larger than 5% was considered as credible.