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. 2018 Nov 2;19(11):3441. doi: 10.3390/ijms19113441

Figure 3.

Figure 3

Example of muscle-specific expression of dCryAB. (A) dCryAB regulatory region contains several binding sites for muscle/mesoderm transcription factors, including TWI, CF2, and Mef2. Others indicated in the scheme binding sites correspond to E74A—Ecdysone-induced protein 74; ERE—Ecdysone response element; Dr—Drop/Msh; HSF—heat shock factor; AP1—Jra transcription factor; DWHN/Jumu—Jumeau; DTCF—Wingless effector; KNI—Knirps. (B) Presented in (A) 5′ dCryAB regulatory region cloned upstream of GFP reporter gene drives expression in somatic muscles. Presented is a lateral view of late stage dCryAB–muscleEnh–GFP embryo. GFP expression is detected in lateral transverse (LT) and a subset of head muscles. A single optical section is shown. Scale bar 40 µm.