Figure 2.

Validation by RT-qPCR analysis of the 10 most significantly up-regulated (A) and the 10 most significantly down-regulated (B) genes identified by microarray experiments after treatment of human T lymphocytes with 2 µM B[α]P for 48 h. Primary human T lymphocytes activated for 72 h were treated with DMSO (CTR) or with 2 µM B[α]P for the last 48 h of culture. mRNA expression was analyzed using RT-qPCR. Data are expressed relative to mRNA expression level in CTR T lymphocytes, arbitrarily set at 1 unit, and are shown as mean ± SEM of 4 independent experiments performed on the same pools of RNAs isolated from CTR or B[α]P-treated cultures as those used in microarray experiments, with triplicate per experiment. * p ≤ 0.05 when compared with CTR T lymphocytes (paired Student’s t test).