Figure 6.

RT-PCR analyses of seven salinity-tolerant candidate genes (IpSR1, IpSR14, IpSR18, IpSR19, IpSR26, IpSR33, and IpSR38) in the roots, vines, and leaves of I. pes-caprae seedlings under salinity (left, 300 mM NaCl) and osmotic (right, 300 mM mannitol) stresses at 0 and 24 h. The house-keeping gene IpUBQ (GenBank accession number: MF502417) was used as a reference gene. All determinations were carried out for three biological replicates. The results shown are the mean ± SD (n ≥ 3). * and ** indicate significant differences in comparison with the wild type at 0.01 < p < 0.05 and p < 0.01, respectively (Student’s t-test).