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. 2018 Nov 16;19(11):3622. doi: 10.3390/ijms19113622

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Inhibitory effects of DGK on inflammatory proteins and cytokines expression in esophagus. The expression levels of IL-1β (a) and TNF-α (b) in esophagus tissue were measured by enzyme-linked immunosorbent assay (ELISA). Expression of pro-inflammatory protein TNF-α, IL-1β, iNOS, COX-2, p-IκB-α, and p-p65 in esophagus tissue were measured by Western blotting (c–i). Rats were pre-treated with DGK 100 and 200 mg/kg body weight excluding normal and RE control for 90 min before surgery, which was performed under inhalation anesthesia. Then, 5 h later, the esophageal tissue was cut, and then, the was abdomen open and removed from each rat. Data are means ± standard deviation (SD). significance: ^## p < 0.01 and ^# p < 0.05 compared with normal control group; ** p < 0.01 and * p < 0.05 compared with RE control group.

Inhibitory effects of DGK on inflammatory proteins and cytokines expression in esophagus. The expression levels of IL-1β (a) and TNF-α (b) in esophagus tissue were measured by enzyme-linked immunosorbent assay (ELISA). Expression of pro-inflammatory protein TNF-α, IL-1β, iNOS, COX-2, p-IκB-α, and p-p65 in esophagus tissue were measured by Western blotting (c–i). Rats were pre-treated with DGK 100 and 200 mg/kg body weight excluding normal and RE control for 90 min before surgery, which was performed under inhalation anesthesia. Then, 5 h later, the esophageal tissue was cut, and then, the was abdomen open and removed from each rat. Data are means ± standard deviation (SD). significance: ^## p < 0.01 and ^# p < 0.05 compared with normal control group; ** p < 0.01 and * p < 0.05 compared with RE control group.