Table 3.
The condition of LC-MS/MS analysis.
| Parameter | Condition | |||
|---|---|---|---|---|
| Ultra-performance liquid chromatography (UPLC) | ACQUITY UPLC HSS T3 | |||
| Column | 100 mm × 2.1 mm, 1.8 µm, Waters | |||
| Column temperature | 40 °C | |||
| Flow rate | 0.5 mL/min | |||
| mobile phase | A (water + 0.1% formic acid) | |||
| B (acetonitrile + 0.1% formic acid) | ||||
| Time | A (%) | B (%) | ||
| 0 | 97 | 3 | ||
| 5 | 97 | 3 | ||
| 16 | 0 | 100 | ||
| 17 | 0 | 100 | ||
| 19 | 97 | 3 | ||
| 25 | 97 | 3 | ||
| injection volume | 5 µL | |||
| metabolite eluted | SYNAPT G2 Si HDMS QTOF (Waters), positive and negative |
|||
| positive | capillary voltage (kV) | 2 | ||
| cone voltage (V) | 40 | |||
| negative | capillary voltage (kV) | 1 | ||
| cone voltage (V) | 40 | |||
| scan range (Da) | 50 to 1200 | |||
| scan time (s) | 0.2 | |||