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. 2018 Nov 18;19(11):3636. doi: 10.3390/ijms19113636

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Growth profile and QS-related phenotype in wild-type, mutant, complicon and overexpressor. (A) Growth and QS-based colorization of biosensor A136; (B) Relative expression level of robp gene encoding right origin-binding protein; (C) Relative expression level of ahl gene encoding acyl homoserine lactone synthase. WT: wild-type (strain T1lg65); robp::Tn: mutant of strain T1lg65 with disrupted robp gene by Tn10; robp::Tn^c: complon of robp::Tn with complementation of robp; WT+robp^c: overexpressor of strain T1lg65 with overexpression of robp. All independent experiments were repeated in triplicate. Significant difference is respectively indicated by asterisks as follows: *** p < 0.001; **** p < 0.0001. Gene expression in WT at 1h was treated as 1 (internal reference).

Growth profile and QS-related phenotype in wild-type, mutant, complicon and overexpressor. (A) Growth and QS-based colorization of biosensor A136; (B) Relative expression level of robp gene encoding right origin-binding protein; (C) Relative expression level of ahl gene encoding acyl homoserine lactone synthase. WT: wild-type (strain T1lg65); robp::Tn: mutant of strain T1lg65 with disrupted robp gene by Tn10; robp::Tn^c: complon of robp::Tn with complementation of robp; WT+robp^c: overexpressor of strain T1lg65 with overexpression of robp. All independent experiments were repeated in triplicate. Significant difference is respectively indicated by asterisks as follows: *** p < 0.001; **** p < 0.0001. Gene expression in WT at 1h was treated as 1 (internal reference).