Figure 2.

NMP decreases sclerostin mRNA and protein expression. (A) Immortalized mouse IDG-SW3 cells were stimulated with 2 mM (dark bars) and 5 mM (light bars) NMP during the whole period of differentiation. (B) Rat osteosarcoma UMR-106 cells were incubated with 2 mM (dark bars) and 5 mM (light bars) NMP over a time period of 24 h. (C) UMR-106 cells were treated for 8 h with the indicated NMP concentrations in normal culture medium. Whole cell lysates were subjected to Western Blot analysis indicating an NMP concentration-dependent reduction in sclerostin expression. The star indicates statistical significance at α = 0.05, while “ns” indicates non-significance at the same threshold.