Figure 3.

Inhibiting the expression of OsVTC1-3 impairs the ability of rice roots to scavenge ROS under salt stress. (A) The O₂⁻ content of OsVTC1-3 RI roots under salt treatment. The rice seedlings were treated with 150 mM NaCl for 1 h and then were stained with NBT to show the content of O₂⁻ in rice root. (B) The H₂O₂ content of OsVTC1-3 RI lines under salt treatment. Control indicates that plants were grown under normal conditions, and NaCl indicates that plants were treated with 150 mM NaCl for five days. The arrow indicates the site where O₂⁻ were stained with NBT and showed a blue deposit. About 40–50 plants were used in each experiment. Bars represent SE (±) of three independent assays, and the asterisk indicates that the results were significantly different from that of WT (** p < 0.01 and * p < 0.05). Significance was evaluated by the t-test.