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Overexpression of tRNAGln(UUG) reverts the HAC1 mRNA splicing phenotype of the modification mutants. The indicated strains carrying either the empty vector (EV) or tRNAGln(UUG) (tQUUG) expressing vector were grown in YNB until OD600 = 1.0 before RNA extraction. HAC1i mRNA levels (before/after TM treatment) of the indicated transformants were quantified. Statistical significance was determined using a two-tailed t-test (* p < 0.05).
